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Related Experiment Videos

Rapid, transcript-specific changes in splicing in response to environmental stress.

Jeffrey A Pleiss1, Gregg B Whitworth, Megan Bergkessel

  • 1Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th Street, Genentech Hall, Room N-374, San Francisco, CA 94143-2200, USA.

Molecular Cell
|September 25, 2007
PubMed
Summary
This summary is machine-generated.

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Splicing of most ribosomal protein genes in yeast is specifically inhibited during amino acid starvation. Gene expression regulation through splicing offers rapid environmental responses, similar to transcription.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Gene Expression Regulation

Background:

  • The core splicing machinery is conserved across species, yet Saccharomyces cerevisiae (yeast) lacks alternative splicing.
  • Introns persist in ~5% of yeast genes, with ribosomal protein-encoding genes (RPGs) being disproportionately represented.
  • The functional significance of retained introns in yeast, particularly in RPGs, remains an open question.

Purpose of the Study:

  • To investigate the hypothesis that splicing of ribosomal protein-encoding genes (RPGs) is regulated under conditions of impaired translation.
  • To explore the role of splicing in dynamic gene expression responses to environmental stress in yeast.

Main Methods:

  • Utilized a microarray-based strategy to monitor splicing efficiency across a large number of yeast genes.

Related Experiment Videos

  • Induction of specific environmental stresses: amino acid starvation and exposure to toxic ethanol levels.
  • Analysis of splicing patterns in response to distinct stress conditions.

    • Main Results:

    • Amino acid starvation rapidly and specifically inhibited the splicing of the majority of RPG transcripts within minutes.
    • Exposure to toxic ethanol levels differentially affected splicing: inhibiting some transcripts while enhancing others.
    • Demonstrated condition-specific regulation of splicing in response to environmental cues.

    Conclusions:

    • Splicing in yeast is not constitutive but can be dynamically regulated in response to environmental changes.
    • Regulated splicing provides a mechanism for rapid and specific gene expression adjustments, analogous to transcriptional regulation.
    • This regulatory layer allows yeast to adapt its proteome efficiently under stress conditions.