Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stringent Response in E. coli01:23

Stringent Response in E. coli

61
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
61
Riboswitches01:56

Riboswitches

8.7K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.7K
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

85
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
85
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

70
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
70
RNA Splicing01:32

RNA Splicing

57.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
57.2K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.6K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mapping of in vivo cleavage sites uncovers a major role for yeast RNase III in regulating protein-coding genes.

eLife·2026
Same author

Phospho-mimic βIII-tubulin rescues microtubule and cardiac defects in Duchenne muscular dystrophy mice.

Journal of molecular and cellular cardiology·2026
Same author

Anxiety Suppressed Prefrontal Cortex Brain Activity: Insights From a Large Sample of Functional Near-Infrared Spectroscopy (fNIRS) Data.

Depression and anxiety·2026
Same author

Unveiling the adaptation mechanisms of symbiotic microbial communities in Glycyrrhiza glabra under extreme environmental conditions.

Journal of applied microbiology·2025
Same author

Dissociation of the nuclear basket triggers chromosome loss in aging yeast.

eLife·2025
Same author

RNase III cleavage sites spread across splice junctions enforce sequential snoRNA processing.

EMBO reports·2025

Related Experiment Video

Updated: Sep 18, 2025

Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

14.2K

Cells resist starvation through a nutrient stress splice switch.

Julie Parenteau1, Jasmine Tsang1, Sara R Downs2

  • 1RNA Group, Département de microbiologie et d'infectiologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec J1E 4K8, Canada.

Nucleic Acids Research
|June 23, 2025
PubMed
Summary

Yeast cells resist starvation by altering spliceosome composition, specifically increasing U1 small nuclear ribonucleoprotein (snRNP). This enhances splicing of key introns, boosting starvation tolerance through a novel spliceosome-driven mechanism.

More Related Videos

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.1K
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.6K

Related Experiment Videos

Last Updated: Sep 18, 2025

Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

14.2K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.1K
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.6K

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Genetics

Background:

  • Introns are crucial in eukaryotic gene expression, often removed via splicing.
  • In yeast, certain introns mediate responses to nutrient stress, but the mechanisms are unknown.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which introns confer starvation resistance in yeast.
  • To investigate the role of spliceosome components, particularly U1 snRNP, in nutrient stress adaptation.

Main Methods:

  • Investigated changes in spliceosome stoichiometry under nutrient depletion.
  • Utilized immunoprecipitation assays to assess spliceosomal component binding to introns.
  • Employed genetic mutations (5' splice site, U1 components) to evaluate functional impacts.

Main Results:

  • Nutrient depletion alters splicing efficiency for distinct intron sets.
  • Increased U1 small nuclear ribonucleoprotein (snRNP) abundance enhances splicing of starvation-tolerance introns.
  • Differential U1 snRNP binding to introns correlates with splicing changes and starvation tolerance.
  • Impaired U1 interactions disrupt starvation resistance.

Conclusions:

  • Spliceosome stoichiometry, driven by U1 snRNP levels, is a key regulator of starvation tolerance in yeast.
  • Selective U1 snRNP recruitment to specific introns adapts cells to nutrient stress.
  • This study reveals a novel mechanism of stress adaptation mediated by RNA splicing.