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Updated: Jul 11, 2026
08:49
Murine Ileocolic Bowel Resection with Primary Anastomosis
Published on: October 29, 2014
Supplementation with high-dose ascorbic acid improves intestinal anastomotic healing.
M H Cevikel1, P Tuncyurek, F Ceylan
1Department of Surgery, Adnan Menderes University, Aydin, Turkey.
Summary
High-dose vitamin C (ascorbic acid) supplementation significantly improved anastomotic healing in rats by increasing collagen and enhancing tissue strength. Further research is needed to confirm clinical benefits.
Area of Science:
- Surgical Research
- Gastrointestinal Surgery
- Nutritional Science
Background:
- Ascorbic acid (vitamin C) is vital for tissue healing.
- Its role in intestinal anastomosis healing is not fully understood.
- This study investigates ascorbic acid's effect on experimental intestinal resection healing.
Purpose of the Study:
- To evaluate the impact of ascorbic acid supplementation on anastomotic healing after intestinal resection in rats.
- To determine if varying doses of ascorbic acid influence healing parameters.
Main Methods:
- Male Wistar albino rats underwent intestinal resection and were divided into three groups: sham, 100 mg/kg ascorbic acid, and 200 mg/kg ascorbic acid.
- Ascorbic acid was administered intramuscularly daily.
- Anastomotic bursting pressure, hydroxyproline levels, and histological analysis were performed on postoperative day 7.
Main Results:
- Higher doses of ascorbic acid significantly increased anastomotic bursting pressure compared to the sham group.
- Hydroxyproline levels, a marker of collagen, were significantly elevated in the high-dose ascorbic acid group.
- Histopathological scores demonstrated improved tissue healing with ascorbic acid supplementation.
Conclusions:
- Supraphysiological doses of ascorbic acid enhance anastomotic healing in experimental intestinal resection.
- Vitamin C supplementation positively influences collagen accumulation, inflammatory response, and anastomotic strength.
- The clinical applicability of high-dose ascorbic acid for anastomotic healing requires further investigation.
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