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Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Related Experiment Video

Updated: Jul 11, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

High-throughput proteomics using antibody microarrays: an update.

Carl A K Borrebaeck¹, Christer Wingren

¹Lund University, Department of Immunotechnology & CREATE Health, BMC D13, SE-221 84 Lund, Sweden. carl.borrebaeck@immun.lth.se

Expert Review of Molecular Diagnostics

|September 26, 2007

Summary

Antibody-based microarrays offer powerful, ultrasensitive protein analysis for disease proteomics. This technology enables detailed serum protein profiling for biomarker discovery and diagnostics.

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Last Updated: Jul 11, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

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Area of Science:

Proteomics
Biotechnology
Molecular Diagnostics

Background:

Antibody-based microarrays are advancing rapidly in disease proteomics.
Miniaturized arrays allow for high-specificity antibody fabrication.
These arrays enable ultrasensitive assays for complex proteomes.

Purpose of the Study:

To provide an update on high-throughput proteomics using antibody-based microarrays.
To focus on technological advances and novel applications in the last 3 years.

Main Methods:

Fabrication of miniaturized micro- and nanoarrays with specific antibodies.
Performing multiplexed and ultrasensitive assays on complex proteomes.
Generating microarray images for protein expression profiling.

Main Results:

Demonstration of antibody-based microarrays in demanding serum protein profiling.
Capability to analyze both high- and low-abundance analytes.
Conversion of microarray data into protein expression profiles or atlases.

Conclusions:

Antibody-based microarrays show great promise in disease proteomics.
The technology offers unique opportunities for diagnostics, biomarker discovery, and drug target identification.
Recent advances enhance capabilities for detailed sample composition analysis.