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Related Concept Videos

Burn Injuries01:22

Burn Injuries

Burn injuries occur when the skin and underlying tissues are damaged due to exposure to heat, electricity, chemicals, radiation, or friction. They can vary in severity, from minor superficial burns to severe deep burns that can be life-threatening.
The damage results in the death of skin cells, which can lead to a massive loss of fluid. Dehydration, electrolyte imbalance, and renal and circulatory failure follow, which can be fatal. Burn patients are treated with intravenous fluids to offset...

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Related Experiment Video

Updated: Jul 11, 2026

Corneal and Limbal Alkali Injury Induction Using a Punch-Trephine Technique in a Mouse Model
07:39

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Published on: August 4, 2023

Histopathologic limbus evolution after alkaline burns.

José Santiago López-García1, Luis Rivas Jara, Isabel García-Lozano

  • 1Ophthalmology Service, Hospital Cruz Roja, Madrid, Spain. docsantilopez@hotmail.com

Cornea
|September 26, 2007
PubMed
Summary

Amniotic membrane transplantation (AMT) enhanced corneal limbal regeneration in moderate alkaline burns. For severe burns, autologous limbal transplantation (ALT) combined with AMT offered the best epithelial and stromal regeneration.

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Last Updated: Jul 11, 2026

Corneal and Limbal Alkali Injury Induction Using a Punch-Trephine Technique in a Mouse Model
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Published on: August 4, 2023

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An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse
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Published on: April 7, 2014

Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Alkaline burns cause severe damage to the corneal limbus, affecting vision.
  • Understanding limbal histopathologic evolution is crucial for effective treatment strategies.

Purpose of the Study:

  • To investigate the histopathologic changes in the corneal limbus following alkaline burns.
  • To evaluate the impact of different therapies on limbal regeneration based on burn severity.

Main Methods:

  • Prospective study of 15 eyes with moderate to severe alkaline burns.
  • Patients received medical therapy, amniotic membrane transplantation (AMT), autologous limbal transplantation (ALT), or ALT + AMT.
  • Limbal biopsies were analyzed immediately after injury and 9 months later.

Main Results:

  • Moderate burns: AMT showed significant stromal and epithelial regeneration compared to medical therapy.
  • Severe burns: AMT resulted in stromal regeneration but incomplete reepithelialization.
  • ALT led to good reepithelialization but defective stromal regeneration.
  • ALT + AMT demonstrated notable epithelial and stromal regeneration in severe burns.

Conclusions:

  • AMT is more effective than medical therapy for limbal stromal and epithelial regeneration in moderate alkaline burns.
  • ALT + AMT provides the optimal outcomes for reepithelialization and stromal regeneration in severe alkaline burns.