Related Experiment Video
Updated: Jul 11, 2026

Determination of the Gas-phase Acidities of Oligopeptides
Published on: June 24, 2013
Energy-dependent kinetic method: application to the multicompetitive fragmentation pathways of protonated peptides
Isabelle Compagnon1, Philippe Dugourd
1Université de Lyon, Université Lyon 1, CNRS, LASIM UMR 5579, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France.
Abstract:
A variation of the kinetic method for the analysis of fragmentation patterns in mass spectra is proposed. The procedure presents three notable features: no evaluation of the effective temperature of the parent ion is required; the ratio of the activation energies for all competitive channels at play are provided; and the measurement is not biased by the mass discrimination of the instrument. The method is based on the analysis of mass spectra recorded as a function of both the excitation energy and the excitation time. Collision-activated dissociation of protonated Leu-enkephalin achieved in a quadrupolar ion trap and analyzed with this method is presented.
Related Concept Videos
Mass Spectrometry: Molecular Fragmentation Overview
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation
For example, the fragmentation of...
Mass Spectrometry: Alkene Fragmentation
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

