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Updated: Jul 10, 2026
08:38
A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy
Published on: April 30, 2015
Basal activity of Kupffer cells increases with old age
Sarah N Hilmer1, Victoria C Cogger, David G Le Couteur
1Centre for Education and Research on Ageing and ANZAC Research Institute, University of Sydney and Concord Hospital, Concord, New South Wales, Australia. shilmer@med.usyd.edu.au
Summary
Kupffer cell numbers and phagocytic activity increase with age in rats, impacting liver function and immune responses. These age-related changes are evident even in the basal state of older animals.
Area of Science:
- Immunology
- Hepatology
- Gerontology
Background:
- Kupffer cells are crucial for hepatic immune surveillance.
- Age-related alterations in Kupffer cell function may affect systemic immunity and liver health.
Purpose of the Study:
- To investigate age-dependent changes in Kupffer cell number and phagocytic function in rats.
- To assess the implications of these changes for hepatic function and immune responses.
Main Methods:
- Isolated perfused rat livers from young, middle-aged, and old rats were used.
- Kupffer cell numbers were quantified using light microscopy.
- Phagocytic activity was assessed by measuring the clearance of microspheres.
Main Results:
- Kupffer cell numbers per field significantly increased with age.
- Phagocytic activity, indicated by reduced microsphere recovery, was higher in older rats.
- Increased Kupffer cell number and activity were observed in the basal state in old rats.
Conclusions:
- Aging leads to increased Kupffer cell numbers and enhanced phagocytic activity in rats.
- These age-related changes in Kupffer cells may significantly influence liver function and systemic immunity.
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