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Updated: Jul 11, 2026

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes
Published on: February 24, 2023
[Fluid shear stress increases the Ca2+ concentration in bone-marrow derived osteoclast-like cells]
Ming Chen1, Xing Liang, Ying Wen
1Dept. of Prosthodontics, Beijing Stomatologicul Hospital, Capital Medical University, Beijing 100050, China.
Objective:
To study the change of Ca2+ density in cultured osteoclast-like cells in response to fluid shear stress.
Methods:
Laser scanning confocal microscope and fluorescent probe were used to detect the free Ca2+ in osteoclast-like cells before and after undergoing fluid shear stress. The images were analyzed and compared with image software.
Results:
At 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM. Compared with contol group, the average intensity of Ca2+ fluorescent signal in osteoclast-like cells undergoing fluid shear stress increased significantly.
Conclusion:
The Cal2+ concentration in bone-marrow derived osteoclast-like cells is sensitive to fluid shear stress, which suggests osteoclast-like cells modulate their function in response to fluid shear stress through the change of free Ca2+ concentration.
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