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Neural localization of addicsin in mouse brain
Saori Akiduki1, Tomoyo Ochiishi, Mitsushi J Ikemoto
1Age Dimension Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Neuroscience Letters
|September 28, 2007
Summary
Addicsin, a protein modulator of neural glutamate transporters, is widely distributed in the mature central nervous system (CNS). Its localization in neurons suggests crucial roles in fundamental CNS physiological functions.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- Addicsin belongs to the prenylated Rab acceptor (PRA) 1 domain family.
- It is a murine homolog of rat glutamate-transporter-associated protein 3-18 (GTRAP3-18).
- Addicsin is hypothesized to modulate the neural glutamate transporter EAAC1, but its functions are largely unknown.
Purpose of the Study:
- To investigate the regional and cellular localization of addicsin in the central nervous system (CNS).
- To elucidate the potential roles of addicsin in CNS physiology.
Main Methods:
- Generation of a novel antibody specific for addicsin.
- Western blot and immunohistochemistry for protein distribution analysis.
- Double immunofluorescence and subcellular fractionation for cellular and synaptic localization.
Main Results:
- Addicsin is widely distributed across various regions of the mature CNS, including the olfactory bulbs, cerebral cortex, amygdala, hippocampus, and cerebellum.
- The protein is expressed in the somata of principal neurons (pyramidal cells) and GABA-ergic interneurons.
- Addicsin exhibits pre-synaptic and cytoplasmic localization within purified synaptic fractions.
Conclusions:
- Addicsin demonstrates significant neural expression in the mature CNS.
- The localization patterns suggest addicsin plays critical roles in fundamental CNS physiological functions.
