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Invasive capabilities of Campylobacter jejuni strains isolated in Bahrain: molecular and phenotypic characterization

Sana A Al-Shaikh1, Abiola C Senok, Abdulrahman Y Ismaeel

  • 1Department of Microbiology, Immunology & Infectious Diseases, College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Kingdom of Bahrain.

Acta Microbiologica Et Immunologica Hungarica
|September 29, 2007
PubMed

Insights

Virulence genes in Campylobacter jejuni correlate with infection severity. The cytolethal distending toxin (cdtB), invasion associated marker (iam), and Campylobacter invasion antigen (ciaB) genes were linked to higher in vitro invasiveness.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • The relationship between Campylobacter jejuni virulence factors and disease severity is not fully understood.
  • Investigating specific genes like cdtB, iam, and ciaB is crucial for understanding C. jejuni pathogenicity.

Purpose of the Study:

  • To characterize three virulence genes (cdtB, iam, ciaB) in C. jejuni clinical isolates.
  • To correlate the presence of these genes with in vitro invasiveness and clinical infection severity.

Main Methods:

  • PCR was used to detect the presence of cdtB, iam, and ciaB genes in eight C. jejuni strains.
  • In vitro invasion assays were performed using INT407 cells to assess bacterial invasiveness.

Main Results:

  • Strains positive for all three genes exhibited the highest invasiveness and were isolated from patients with severe infections.
  • A correlation was observed between the presence of all three genes and enhanced in vitro invasive capability.
  • The ciaB gene appeared to be a significant contributor to the invasive phenotype.

Conclusions:

  • The presence of cdtB, iam, and ciaB genes in C. jejuni is associated with increased in vitro invasiveness.
  • The Campylobacter invasion antigen (ciaB) gene may play a key role in conferring invasive properties to C. jejuni.

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