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Updated: Jul 11, 2026

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

Mapping protein post-translational modifications with mass spectrometry.

Eric S Witze¹, William M Old, Katheryn A Resing

¹Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado at Boulder, Boulder, Colorado 80309-0215, USA.

|September 29, 2007

Summary

This review explores mass spectrometry techniques for identifying protein post-translational modifications. Understanding these chemical changes is key to unlocking cellular regulation mechanisms.

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Area of Science:

Biochemistry
Molecular Biology
Proteomics

Background:

Post-translational modifications (PTMs) are crucial for protein function and cellular regulation.
Understanding the diversity and dynamics of PTMs is essential for deciphering complex biological processes.

Purpose of the Study:

To provide biologists with an overview of current mass spectrometry-based proteomics strategies.
To detail methods for characterizing protein post-translational modifications.
To discuss the strengths and limitations of various proteomic approaches for PTM analysis.

Main Methods:

Mass spectrometry (MS) is presented as a core technology for PTM detection and quantification.
Large-scale proteomic experiments enabling the screening of complex protein mixtures for modifications are highlighted.
Strategies for profiling protein chemistry to understand biological control are discussed.

Main Results:

Modern MS approaches facilitate comprehensive analysis of PTMs.
The review synthesizes current knowledge on mass spectrometry applications in PTM research.
Insights into the strengths and weaknesses of different techniques are provided.

Conclusions:

Mass spectrometry-based proteomics offers powerful tools for characterizing protein PTMs.
This review equips biologists with knowledge to select appropriate MS strategies for PTM studies.
Advancements in MS are critical for deeper insights into cell regulation via protein modifications.