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Published on: April 20, 2018
Activity-based protein profiling for the functional annotation of enzymes
Katherine T Barglow1, Benjamin F Cravatt
1The Skaggs Institute for Chemical Biology and Department of Chemical Physiology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, California 92037, USA.
Nature Methods
|September 29, 2007
Summary
Activity-based protein profiling (ABPP) uses chemical probes to track enzyme activity in biological systems. This technology helps assign functions to uncharacterized enzymes in proteomes.
Area of Science:
- Biochemistry and Molecular Biology
- Proteomics
- Chemical Biology
Background:
- Activity-based protein profiling (ABPP) is an emerging post-genomic technology.
- It utilizes active site-directed chemical probes to monitor enzyme function.
- ABPP probes have been developed for various enzyme classes.
Purpose of the Study:
- To demonstrate the utility of ABPP as a tool for functional enzyme annotation.
- To showcase how ABPP can be applied to understand enzyme activities in complex biological systems.
- To highlight the potential of ABPP in assigning functions to uncharacterized enzymes.
Main Methods:
- Development and application of active site-directed chemical probes.
- Utilizing ABPP to profile enzyme activities in complex biological samples.
- Analyzing proteomes to identify and characterize enzyme functions.
Main Results:
- ABPP probes have been successfully developed for multiple enzyme classes.
- Enzyme activities have been inventoried en masse across various physiological and pathological processes.
- Specific examples illustrate the application of ABPP in functional enzyme assignment.
Conclusions:
- ABPP provides a powerful set of chemical tools for functional proteomics.
- The technology facilitates the assignment of functions to uncharacterized enzymes in both eukaryotic and prokaryotic proteomes.
- ABPP is a valuable approach for advancing our understanding of enzyme roles in biological systems.
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