Related Experiment Video
Updated: Jul 11, 2026
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
Published on: February 21, 2019
Electron transfer in SAM/cytochrome/polyelectrolyte hybrid systems on electrodes: a time-resolved surface-enhanced
Jana Grochol1, Roman Dronov, Fred Lisdat
1Institut für Chemie, Technische Universität Berlin, Sekr. PC14, Strasse des 17. Juni 135, D-10623 Berlin, Germany.
Abstract:
Silver electrodes were covered with mixed self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA) and 11-mercaptoundecanol (MU) and subsequently coated with alternating layers of cytochrome c (Cyt) and poly(anilinesulfonic acid) (PASA). The immobilized protein is electroactive and retains its native structure. Compared to the case of systems on gold electrodes, the stability of the assembly was found to be decreased. The redox process of Cyt is accompanied by reversible oxidation-reduction of PASA as revealed by the comparative surface-enhanced resonance Raman (SERR) analysis of assemblies including Cyt and the redox-inactive apo-cytochrome c. Time-resolved SERR experiments show a fast electron exchange between the protein and the polyelectrolyte that may play a supporting role in the electric communication of thicker multilayer assemblies employed as sensors.
Related Concept Videos
01:26Photochemical Electrocyclic Reactions: Stereochemistry
Selection Rules: Photochemical Activation
01:19Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...