Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation and counting of multiple cell types using an affinity separation device.

Kelong Wang1, Brandon Cometti, Dimitri Pappas

  • 1Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409-1061, USA.

Analytica Chimica Acta
|October 2, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design, synthesis, and analysis of multi-layered 3D fluorescent polymers derived from anthracene and naphthalene structural units.

RSC advances·2026
Same author

ZDHHC9 and spermine metabolism: a palmitoylation-driven pathway to prostate carcinogenesis.

Journal of translational medicine·2025
Same author

A highly sensitive ESIPT fluorescent probe for visualization of HK2-mediated lung cancer progression via imaging nitroreductase.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025
Same author

Small-molecule fluorescent probes for imaging intracellular amyloid toxicity induces ferroptosis via HClO fluctuation in Alzheimer's disease.

Analytica chimica acta·2025
Same author

Isolation and detection of target cells in blood <i>via</i> immunomagnetic separation and atomic emission spectroscopy.

Analytical methods : advancing methods and applications·2025
Same author

Evaluating antibiofilm efficacy of carbon dots againstPseudomonas Aeruginosabased on precursor molecule selection.

Colloids and surfaces. B, Biointerfaces·2025
Same journal

Programmable DNA probe-mediated nanopore biosensor for multiplex nucleic acid detection and its application in milk authenticity identification.

Analytica chimica acta·2026
Same journal

A multifunctional fluorescent sensor for sequential off-on-off visual detection of Zn<sup>2+</sup> and glyphosate in food and biological matrices and efficient removal of Zn<sup>2+</sup> from aqueous media.

Analytica chimica acta·2026
Same journal

Automated carousel-based electrochemical sensing toward microbiological and oncological settings.

Analytica chimica acta·2026
Same journal

Label-free quantification of cumulative cytosol-enriched peptide concentrations by mass spectrometry.

Analytica chimica acta·2026
Same journal

Integrated multi-matrix bile acid metabolic metrics (BAMMs): A methodological framework for functional metabolic phenotyping in human subjects.

Analytica chimica acta·2026
Same journal

A dual-enzymatic activity/SERS dual-mode sensor array based on BSA-Cu nanoflowers for sensitive detection of various foodborne pathogens.

Analytica chimica acta·2026
See all related articles

This study introduces a novel, label-free cell separation device using antibody-coated chips for accurate T and B lymphocyte enumeration. This simple method offers rapid analysis and minimal sample preparation, ideal for remote settings.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Immunology

Background:

  • Accurate cell enumeration is crucial for diagnostics and research.
  • Existing cell separation methods often require complex labeling and analysis.
  • A need exists for simple, rapid, and label-free cell separation techniques.

Purpose of the Study:

  • To describe a simple device for label-free cell separation and enumeration.
  • To validate the device's performance using T and B lymphocytes.
  • To assess the potential of this technology for remote applications.

Main Methods:

  • Development of a glass chip device utilizing capture antibodies for cell isolation.
  • Label-free readout performed using transmission microscopy.
  • Separation and enumeration of T and B lymphocytes (CD4, CD19, CD71 antigens) from various samples.

Related Experiment Videos

Main Results:

  • The device reproducibly binds cells based on antigen expression, enabling accurate enumeration.
  • Inter- and intra-device variability were assessed, and nonspecific binding was addressed.
  • Rapid analysis (20 min) with minimal sample preparation was achieved.

Conclusions:

  • The developed device offers a simple, label-free method for cell separation and enumeration.
  • The technology demonstrates potential for use in resource-limited or remote settings.
  • This approach simplifies cell analysis, reducing complexity and time.