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Updated: Jul 11, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Published on: March 15, 2017
Strong ion-exchange centrifugal partition chromatography as an efficient method for the large-scale purification of
Alix Toribio1, Jean-Marc Nuzillard, Jean-Hugues Renault
1FRE CNRS 2715, IFR 53 Biomolecules, UFR Pharmacie, Université de Reims Champagne-Ardenne, Bât. 18, Moulin de Housse, B.P. 1039, 51687 Reims Cedex 2, France.
Abstract:
The glucosinolates sinalbin and glucoraphanin were purified by strong ion-exchange displacement centrifugal partition chromatography (SIXCPC). The optimized conditions involved the biphasic solvent system ethyl acetate/n-butanol/water (3:2:5, v/v), the lipophilic anion-exchanger Aliquat 336 (trioctylmethylammonium chloride, 160 and 408 mM) and a sodium iodide solution (80 and 272 mM) as displacer. Amounts as high as 2.4 g of sinalbin and 2.6g of glucoraphanin were obtained in one step in 2.5 and 3.5h respectively, starting from 12 and 25 g of mustard and broccoli seed aqueous extracts, using a laboratory scale CPC column (200 mL inner volume).
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