[Mapping and genetic effect analysis of quantitative trait loci related to body size in common carp (Cyprinus carpio...

The common carp recombinant inbred lines (RIL) derived from the cross Barbless carp x Hebao-cold tolerance red carp were used as experimental materials in this study. Based on the linkage map constructed with simple sequence repeat (SSR) markers using this RIL population, marker regression and complexity interval mapping were analyzed by Windows Map Manager2.0 software. A P-value of 0.01 was the threshold value of single-marker. A linkage group-wide permutation test (1 000 replicates) determined the significance of the maximum LOD value over the various intervals analyzed for each linkage group. The results are as follows: 1) For maker regression, a total of 7 makers related body size, were at 1% significant level, of which 3 were at 0.1% significant level. The variance explained by these loci, ranged 14.00% to 27.00%. Loci HLJ534, HLJ319, and HLJ370 at 0.1% significant level closely linked to the gene related to body size of common carp; 2) six significant QTLs related to body size were at the 5% linkage group-wide level on these linkage groups, and two of them were at 1% level. The variances explained by these QTLs ranged from 11.33% to 23.12% and their additive effects were not identical. HLJ190-HLJ497 and HLJ479-HLJ483 were major QTLs associated to body size of common carp.