[A study of the metabolism of bacterial cells by the method of surface plasmon spectroscopy]

The metabolism of bacterial cells has been studied by the method based on the excitation surface plasmon resonance (SPR) in structures metal-buffer layer-bacterial monolayer. The possibility of monitoring the dynamics of metabolism in the mode of real time was shown. Changes in the volume of a bacterial monolayer during the nutrition of cells were revealed. The dependences of change in the volume of cells on the quantity of substance necessary for the metabolic process were found. It was established that the cyclic presence of gas participating in the metabolism leads to reversive changes in the properties of bacteria in the monolayer.