Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antioxidants block cyclic loading induced chondrocyte death.

B R Beecher1, J A Martin, D R Pedersen

  • 1Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA 52242, USA.

The Iowa Orthopaedic Journal
|October 3, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced phagocytic capacity endows chondrogenic progenitor cells with a novel scavenger function within injured cartilage.

Osteoarthritis and cartilage·2016
Same author

Clinical outcomes of patellar chondral lesions treated with juvenile particulated cartilage allografts.

The Iowa orthopaedic journal·2014
Same author

Single cell sorting identifies progenitor cell population from full thickness bovine articular cartilage.

Osteoarthritis and cartilage·2014
Same author

Hinged total elbow replacement.

Orthopedics·2014
Same author

Architecture of the periacetabular trabecular bone.

Orthopedics·2014
Same author

Hematogenous proteus mirabilis osteomyelitis.

Orthopedics·2014

High joint loading can cause cartilage damage and cell death, potentially leading to osteoarthritis. Antioxidants may protect cartilage cells from this damage, suggesting new prevention strategies.

Area of Science:

  • Biomedical Engineering
  • Orthopedics
  • Cell Biology

Background:

  • Articular cartilage normally functions under cyclic loading.
  • Joint incongruities can cause localized stress, leading to chondrocyte (cartilage cell) damage.
  • This damage may initiate osteoarthritis (OA) through chondrocyte attrition.

Purpose of the Study:

  • To investigate if oxidants mediate stress-induced chondrocyte apoptosis.
  • To determine if antioxidants can reduce chondrocyte attrition from excessive joint loading.

Main Methods:

  • Human articular cartilage explants underwent cyclic axial compression at 2 MPa and 5 MPa.
  • Chondrocyte death and lactate accumulation were measured.
  • The effects of antioxidants (vitamin E, NAC, SOD) on cell death and lactate levels were assessed.

Related Experiment Videos

Main Results:

  • 5 MPa compression caused 30% chondrocyte death; 2 MPa caused <15%.
  • High compression suppressed lactate secretion, indicating metabolic stress.
  • Antioxidants blocked ~80% of cell death and prevented lactate suppression.

Conclusions:

  • Oxidative stress mediates harmful effects of abnormal joint loading on chondrocytes.
  • Antioxidant treatments show potential for preventing osteoarthritis by minimizing oxidative damage.