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Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
Published on: July 26, 2019
Experimental approach for assessing the outcome accuracy of antibody microarray experiments
Qiang Gu1, Thamil Mani Sivanandam, Jessica Haymore
1Department of Neurobiology and Anatomy, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA. qgu@wfubmc.edu
Journal of Proteome Research
|October 4, 2007
Summary
A new quality control strategy for antibody microarray analysis is proposed. This method uses existing proteins, simplifying quality assessment for antibody microarrays and improving experimental accuracy.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Genomics
Background:
- Antibody microarrays are crucial for high-throughput proteomic analysis.
- Ensuring the accuracy and reliability of antibody microarray data is essential for reproducible research.
- Current quality control methods can be complex and require specialized reagents.
Purpose of the Study:
- To develop a simplified and effective experimental strategy for quality control in antibody microarray analyses.
- To establish a method that does not rely on exogenous reference markers or absolute protein quantification.
- To provide a practical tool for assessing the accuracy of antibody microarray experiments.
Main Methods:
- The proposed method utilizes proteins already prepared for standard antibody microarray experiments.
- It avoids the need for external positive or negative control markers.
- It does not require the determination of absolute protein concentrations within the sample.
Main Results:
- Validation experiments confirmed the fundamental principle of the proposed quality control approach.
- The method demonstrated its feasibility for routine application in antibody microarray workflows.
- The strategy effectively assesses the outcome accuracy of the analyses.
Conclusions:
- The developed experimental strategy offers a robust and user-friendly approach to antibody microarray quality control.
- This method enhances the reliability and reproducibility of antibody microarray data.
- It serves as a valuable tool for validating the accuracy of proteomic profiling experiments.
