Related Experiment Video
Updated: Jul 11, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
Published on: February 8, 2014
Focus detection from digital in-line holograms based on spectral l1 norms
Weichang Li1, Nick C Loomis, Qiao Hu
1Applied Ocean Physics and Engineering Department, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, USA. lwc@alum.mit.edu
Abstract:
A rapid focus-detection technique based directly on the spectral content of digital holograms is developed. It differs from previous approaches in that it does not need a full reconstruction of the image. The technique uses l(1) norms of object spectral components associated with the real and imaginary parts of the reconstruction kernel. Further, the l(1) norms can be computed efficiently in the spatial frequency domain using a polar coordinate system, yielding a drastic speedup of approximately 2 orders of magnitude compared with image-based focus detection. Significant computational savings are achieved when subsequent image reconstructions are done selectively over the detected focus distances. Focus-detection results from holograms of plankton are demonstrated that show the technique is both accurate and robust.
Related Concept Videos
Difference from Background: Limit of Detection
The LOD indicates the presence or absence...
Detection of Black Holes
Their closest cousins are neutron stars, which are composed almost entirely of neutrons packed against each other, making them extremely dense. A neutron star has the same mass as the Sun but its diameter is only a few kilometers. Therefore, the escape velocity from their surface is close to the speed of light.
Not until the 1960s, when the first neutron...
IR Frequency Region: Fingerprint Region
The...
High-Performance Liquid Chromatography: Types of Detectors

