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Updated: Jul 11, 2026
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
[Inhibitory effect of Isorhamnetin and Hesperidin on LDL oxidation induced by Cu2+]
1Division of Peptides Related with Human Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China. liurui60@hotmail.com
Objective:
To evaluate the inhibitory effect of Isorhamnetin and Hesperidin on the LDL oxidation induced by Cu2+.
Methods:
The serum LDL was isolated by the one step density gradient ultracentrifugation. The LDL oxidation was induced by Cu2+ in vitro for different time periods. Isorhamnetin and Hesperidin at 5 micromol/L were added respectively, as the experimental groups, 3 hours before oxidation. The oxidation of LDL in experimental and control groups was identified by measuring A234 , REM, TBARS and protein carbonyls content.
Results:
The values of A234, REM, TBARS and protein carbonyls formation increased gradually during LDL oxidation induced by Cu2+ in vitro. During LDL oxidation induced by Cu2+ in vitro and incubation with each of Isorhamnetin and Hesperidin, the kinetic changes of A234 , REM, TBARS and protein carbonyls formation showed lag phases of 2-4 h and 2 h respectively, and the corresponding values for each of the agents treated group were reduced by 23.5%-40.4%, 20.5%-37.7%, 18.6-30.3% and 20.1%-52.4% (P < 0.001); and 11.1%-21.2%, 9.2%-28.3%, 13.7%-21.3% and 5.0%-43.8% respectively (P < 0.001, P < 0.01, P < 0.001 and P < 0.05).
Conclusion:
It suggests that Isorhamnetin and Hesperidin can substantially inhibit LDL oxidation, and Isorhamnetin has antioxidation ability stronger than Hesperidin.
