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Related Experiment Video

Updated: Jul 11, 2026

Isolating Potentiated Hsp104 Variants Using Yeast Proteinopathy Models
08:44

Isolating Potentiated Hsp104 Variants Using Yeast Proteinopathy Models

Published on: November 11, 2014

Hsp72 release: mechanisms and methodologies.

Alexzander Asea1

  • 1Division of Investigative Pathology, Scott & White Clinic and Texas A&M University System Health Science Center College of Medicine, 1901 South 1st Street, Temple, TX 76504, USA. aasea@swmail.sw.org

Methods (San Diego, Calif.)
|October 9, 2007
PubMed
Summary

Heat shock proteins (HSP) are released from cells via passive or active mechanisms. This study explores how stress releases HSP72 into circulation and its role in host defense.

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Last Updated: Jul 11, 2026

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08:44

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Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
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Intracellular Refolding Assay
07:18

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Published on: January 24, 2012

Area of Science:

  • Cellular Biology
  • Immunology
  • Stress Response

Background:

  • Two primary mechanisms govern heat shock protein (HSP) release: passive (necrosis, trauma, surgery, viral infection) and active (non-classical pathway via exosomes or free HSP).
  • Understanding HSP release mechanisms is crucial for comprehending cellular stress responses and immune modulation.

Purpose of the Study:

  • To detail experimental methods for investigating stress-induced release of HSP72 into circulation.
  • To explore the biological significance of circulating HSP72 in host defense against diseases.

Main Methods:

  • Development of experimental procedures to quantify HSP72 release under stress conditions.
  • Analysis of the non-classical protein release pathway involving exosomes and free HSP.

Main Results:

  • Established methodologies to study HSP72 mobilization into the bloodstream following cellular stress.
  • Identified circulating HSP72 as a key factor in host defense mechanisms.

Conclusions:

  • Stress significantly impacts HSP72 release into circulation through both passive and active pathways.
  • Circulating HSP72 plays a vital role in the host's ability to combat disease, highlighting its immunomodulatory functions.