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Updated: Jul 11, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Spectral study of the interaction of DNA with benzothiazolyl-benz-alpha-chromene
V S Sibirtsev1, A V Garabadzhiu
1Mendeleyev Russian Research Institute for Metrology, St. Petersburg, 190005, Russia. vs1969r@mail.ru
Abstract:
Absorption and luminescence excitation and emission spectra of newly synthesized 2-(4-methylphenylimino)-3-(2 -benzothiazolyl)benz-alpha-chromene (BCBT) have been studied in the presence of various DNA concentrations. BCBT is characterized by the existence of two different fluorescent systems, exhibiting radiationless fluorescence resonance energy transfer between them. In the range of molar ratios of polynucleotide/dye concentrations from 0 to 50, BCBT preferentially intercalates into DNA due to its benz-alpha-chromene fragment, whereas the 2-benzothiazolyl fragment is responsible for fluorescence.
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