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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

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Related Experiment Video

Updated: Dec 27, 2025

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

New Method of Integrated Photofluorescence Microbiotesting.

V S Sibirtsev¹, A V Garabadgiu², V I Shvets^2,3

¹All-Russia Research Institute for Food Additives- Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS, 191014, St. Petersburg, Russia. vs1969r@mail.ru.

Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections

|March 5, 2020

Summary

This summary is machine-generated.

This study introduces a rapid biotesting method to assess chemical impacts on microorganisms. It analyzes light scattering, absorption, and fluorescence to evaluate effects on reproduction, metabolism, and genome structure.

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Area of Science:

Microbiology
Biophysics
Biochemistry

Background:

Standard microbiotesting methods are often slow and subjective.
Assessing the impact of chemical factors on microorganisms requires comprehensive analysis.

Purpose of the Study:

To describe a novel biotesting procedure for analyzing microbial responses to chemical factors.
To present the application of this technique for evaluating the antibiotic activity of metal cations.

Main Methods:

Monitoring changes in elastic light scattering, light absorption, and intrinsic photofluorescence of the protein component.
Determining concentration and structuring coefficients of the genomic component in unicellular organisms.
Incubating test organisms in a liquid nutrient medium with and without chemical factors.

Main Results:

The technique allows for rapid, objective, and comprehensive assessment of chemical effects.
Analysis of antibiotic activity of various metal cations was successfully performed.
Observed changes in protein and genomic components indicate effects on reproduction, metabolism, and genome structure.

Conclusions:

The described biotesting procedure offers a significant advancement over traditional methods.
This method provides a more efficient and detailed evaluation of chemical impacts on microbial life.
It is applicable for analyzing various products and wastes for their biological effects.