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Related Experiment Videos

Hollow-fibre affinity cell separation.

Robert E Nordon1, Scott Craig

  • 1Graduate School of Biomedical Engineering, University of New South Wales, 2052 Sydney, Australia. r.nordon@unsw.edu.au

Advances in Biochemical Engineering/Biotechnology
|October 10, 2007
PubMed
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Hollow-fibre affinity cell separation uses monoclonal antibodies immobilized on fibres to separate cells based on adhesion strength. This cost-effective technology offers flexibility and scalability for cell and tissue engineering applications.

Area of Science:

  • Cell and tissue engineering
  • Biophysical processes
  • Biomaterials science

Background:

  • Developing cell and tissue engineering fields require cost-effective cell delivery technologies.
  • Hollow-fibre affinity cell separation is a promising technique for cell fractionation.
  • Monoclonal antibodies serve as ligands immobilized on hollow fibre substrates.

Purpose of the Study:

  • To present the biophysical basis of ligand-mediated cell adhesion in affinity separation.
  • To relate cell adhesion principles to affinity cell separation performance.
  • To discuss hollow fibre hydrodynamics and polymer substrate modification.

Main Methods:

  • Immobilization of monoclonal antibodies (ligands) onto hollow fibre substrates.
  • Fractionation of cells based on adhesion strength using defined shear stress.

Related Experiment Videos

  • Modification of polymer substrates with protein ligands.

    • Main Results:

    • Demonstrated the biophysical principles governing ligand-mediated cell adhesion.
    • Related adhesion strength to cell recovery performance in hollow fibre systems.
    • Discussed hydrodynamics and substrate modification for improved separation.

    Conclusions:

    • Hollow-fibre affinity cell separation provides a flexible and scalable platform for cell delivery.
    • Cost-effectiveness is a key consideration for large-scale epitope-selective cell separation.
    • This technology holds promise for advancing cell and tissue engineering.