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Cell partitioning in aqueous two-phase polymer systems.

J M S Cabral1

  • 1Institute for Biotechnology and Bioengineering, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. joaquim.cabral@ist.utl.pt

Advances in Biochemical Engineering/Biotechnology
|October 10, 2007
PubMed
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This review explores using aqueous two-phase systems with biocompatible polymers for whole cell separation and isolation. It details factors influencing cell partitioning and applications in cell processing and bioconversions.

Area of Science:

  • Biotechnology
  • Separation Science
  • Polymer Chemistry

Background:

  • Aqueous two-phase systems (ATPS) offer a biocompatible method for cell separation.
  • Biocompatible polymers are key to developing effective ATPS for biological applications.

Purpose of the Study:

  • To review whole cell separation and isolation using ATPS based on biocompatible polymers.
  • To analyze physicochemical factors influencing phase separation and cell partitioning.
  • To describe applications of ATPS in cell processing and bioconversions.

Main Methods:

  • Analysis of physicochemical factors affecting ATPS properties.
  • Evaluation of cell partitioning based on polymer characteristics (molecular weight, concentration) and environmental conditions (temperature, pH, ionic species).

Related Experiment Videos

  • Review of affinity extraction techniques and cell surface properties.

    • Main Results:

    • Physicochemical factors significantly influence phase separation and system properties in ATPS.
    • Polymer molecular weight, concentration, temperature, pH, and ionic species are critical for cell partitioning.
    • Stimuli-responsive polymers and affinity extraction enhance ATPS efficiency.

    Conclusions:

    • ATPS provide a versatile platform for cell separation and isolation.
    • Understanding factors influencing cell partitioning is crucial for optimizing ATPS.
    • Emerging applications include continuous cell partitioning in microdevices and extractive bioconversions for the biomedical sector.