Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Channel Rhodopsins

Channel Rhodopsins

Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...

Variables Affecting Phosphorescence and Fluorescence

Variables Affecting Phosphorescence and Fluorescence

Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Blood culture collection practice prior to empiric piperacillin/tazobactam in patients requiring hospitalization: a potential target for antimicrobial stewardship from a single-center retrospective observational study.

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy·2026

Same author

COVID-19-related health literacy, general health literacy, and mental health problems: evidence from a population-based study in Japan.

Environmental health and preventive medicine·2026

Same author

Community-based health-focused longitudinal aging studies in East and Southeast Asia: landscape and future directions.

The Lancet regional health. Western Pacific·2026

Same author

Association between haemodynamics during cardiopulmonary resuscitation and cerebral arterial enhancement.

Resuscitation plus·2026

Same author

Electromagnetic-Guided Nasogastric Tube Insertion by Nurses: A Multicenter Non-Inferiority Study.

Annals of nutrition & metabolism·2026

Same author

Risk factors for in-hospital mortality in elderly patients receiving veno-venous extracorporeal membrane oxygenation: A single-center retrospective study.

Heart & lung : the journal of critical care·2026

Search research articles

Related Experiment Video

Updated: Jul 11, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Linear and nonlinear optical responses in bacteriochlorophyll a.

Mitsuru Sugisaki¹, Ritsuko Fujii, Richard J Cogdell

¹Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi, Osaka, 558-8585, Japan. mitsuru@sci.osaka-cu.ac.jp

Photosynthesis Research

|October 11, 2007

Summary

Nonlinear optical responses of bacteriochlorophyll a were studied using four-wave mixing. A theoretical model including solvent dynamics accurately explained the experimental results, revealing insights into light-harvesting mechanisms.

More Related Videos

Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats

Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats

Published on: October 26, 2019

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Published on: November 6, 2018

Related Experiment Videos

Last Updated: Jul 11, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats

Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats

Published on: October 26, 2019

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus

Published on: November 6, 2018

Area of Science:

* Molecular spectroscopy
* Quantum optics
* Biophysics

Background:

* Bacteriochlorophyll a (BChl a) is a key pigment in photosynthetic light-harvesting complexes.
* Understanding its nonlinear optical properties is crucial for elucidating energy transfer mechanisms.
* Previous studies often employed simplified models that may not capture the full complexity of BChl a's optical response.

Purpose of the Study:

* To investigate the nonlinear optical responses of BChl a using the three-pulse four-wave mixing (FWM) technique.
* To develop and apply a theoretical model that accurately describes these responses.
* To determine the spectral density function governing the optical signals.

Main Methods:

* Experimental investigation using the three-pulse four-wave mixing (FWM) technique.
* Resonant excitation into the Q(y) band of BChl a.
* Theoretical model calculations incorporating the Brownian oscillation mode of the solvent.

Main Results:

* The experimental FWM results were successfully explained by the theoretical model.
* The spectral density function, crucial for optical signal calculation, was determined.
* A simple two-level model was insufficient to account for the observed nonlinear optical responses.

Conclusions:

* The study provides a comprehensive understanding of BChl a's nonlinear optical properties.
* Solvent dynamics play a significant role in shaping the optical signals.
* Higher-order interactions are essential for accurately modeling BChl a's behavior.