Related Experiment Video
Updated: Jul 11, 2026
13:52
Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
Published on: March 19, 2014
High-level expression of recombinant active human renin in Sf-9 cells: rapid purification and characterization
Saori Takahashi1, Keishi Hata, Ken-Ichi Kikuchi
1Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center, Japan. saori@arif.pref.akita.jp
Bioscience, Biotechnology, and Biochemistry
|October 12, 2007
Abstract:
Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%.
