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Related Experiment Video

Updated: Jul 11, 2026

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

Pseudo internal standard approach for label-free quantitative proteomics.

Tsuyoshi Tabata1, Toshitaka Sato, Junro Kuromitsu

  • 1Eisai Co., Ltd. Laboratory of Core Technology, Tokodai 5-1-3, Tsukuba, Ibaraki 300-2635, Japan.

Analytical Chemistry
|October 13, 2007
PubMed
Summary

This study introduces a novel label-free quantitative proteome analysis using pseudo internal standards (PISs). This method simplifies quantitative mass spectrometry by using stable proteins as internal references, avoiding costly isotope labeling.

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Area of Science:

  • Biochemistry
  • Proteomics
  • Analytical Chemistry

Background:

  • Quantitative proteome analysis is crucial for understanding biological functions.
  • Stable isotope labeling is reliable but expensive and time-consuming.
  • Existing label-free methods lack reliability, especially for small molecules.

Purpose of the Study:

  • To develop a novel, simple, and cost-effective label-free quantitative proteome analysis method.
  • To establish a reliable alternative to isotope labeling in mass spectrometry.
  • To adapt the pseudo internal standard (PIS) concept for quantitative proteomics.

Main Methods:

  • Developed a label-free quantitative proteome analysis approach utilizing pseudo internal standards (PISs).
  • Inspired by northern blotting, PISs leverage consistently expressed proteins as internal references.

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Quantitative Analysis of Chromatin Proteomes in Disease
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Last Updated: Jul 11, 2026

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
08:29

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Published on: April 19, 2019

Quantitative Analysis of Chromatin Proteomes in Disease
08:11

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

  • No additional standards or labeling reagents are required.

    • Main Results:

    • The PIS method offers a simple and cost-effective alternative to traditional quantitative proteome analysis.
    • Demonstrated the feasibility of using endogenous, non-changing proteins as internal standards.
    • The approach bypasses the need for expensive and time-consuming isotope labeling.

    Conclusions:

    • The PIS method provides a novel and accessible approach for mass spectrometry-based quantitative proteomics.
    • This technique enhances the reliability of label-free quantitation.
    • The PIS methodology may also be applicable to quantitative metabolome analysis.