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Published on: May 11, 2020
A multiple single nucleotide polymorphisms interrogation assay for reliable Potato virus Y group and variant
Mathieu Rolland1, Laurent Glais, Camille Kerlan
1INRA, Agrocampus Rennes, UMR1099 BiO3P (Biology of Organisms and Populations Applied to Plant Protection), F-35653 Le Rheu, France.
Journal of Virological Methods
|October 13, 2007
Summary
A new SNaPshot assay accurately classifies Potato virus Y (PVY) isolates, including PVYN and PVYO groups and variants. This method improves PVY diagnostics by targeting key genetic markers for reliable identification.
Area of Science:
- Plant virology
- Molecular diagnostics
- Genetics
Background:
- Potato virus Y (PVY) classification relies on biological properties, but existing detection tools may misclassify isolates.
- Current PVY detection methods targeting non-biological markers can lead to inaccurate classification.
Purpose of the Study:
- To develop an improved PVY detection and characterization tool.
- To enhance the accuracy of PVY isolate classification using molecular markers.
Main Methods:
- Development of a single nucleotide polymorphism (SNaPshot) assay.
- Targeting specific PVY polymorphic nucleotides (T/C9259, A/C2271, G/C8573, A/G2213).
- Utilizing four-digit codes (TAGA, CCCG, CACA, CAGA) for isolate identification.
Main Results:
- The SNaPshot assay reliably identifies PVYN, PVYO, PVYN-W, and PVYNTN isolates.
- Efficient detection and characterization of PVY with as few as 10(2) viral RNA copies.
- Accurate PVY group assignment possible with 10 viral RNA genome copies.
- Dual PVY(N)/PVYO characterization in mixed samples (ratios 0.1-10).
Conclusions:
- The SNaPshot assay offers an efficient and reliable method for PVY detection and classification.
- This tool simultaneously targets functional markers and sequences, improving upon previous PVY diagnostic assays.
- The developed assay enhances PVY diagnostics by providing accurate classification of isolates and mixed samples.
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