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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
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Direct Xylella fastidiosa whole genome sequencing from various plant species using targeted enrichment
Anne-Laure Boutigny1, Benoit Remenant1, Bruno Legendre1
1Anses, Plant Health Laboratory, Bacteriology Virology GMO Unit, 7 rue Jean Dixméras, 49044 Angers cedex 01, France.
Journal of Microbiological Methods
|April 7, 2023
Abstract:
A targeted enrichment method was developed to sequence Xylella fastidiosa genomic DNA directly from plant samples. The method was evaluated on various plant species infected with different strains at different levels of contamination. After enrichment, X. fastidiosa genome coverage was above 99.9% for all tested samples.
