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Updated: Jul 10, 2026

In Vivo Confocal Microscopy in the Diagnosis and Management of Dry Eye: A Focus on Imaging Protocols and Interpretation
Published on: November 11, 2025
Depth of focus: clinical manifestation
1Department of Ophthalmology, National Medical Academy of Postgraduate Education, Kiev, Ukraine. nms@micro.kiev.ua
Purpose:
To assess depth of focus on the basis of measurements of the point spread function, which involve Stiles and Crawford phenomenon.
Setting:
Department of Ophthalmology of National Academy of Postgraduate Education.
Methods:
Gullstrand's schematic eye parameters and data of subjective measurement of size of diffusion circles were used for calculation of depth of focus. Data of 1171 eyes with visual acuity 1.0-2.0 were included in the study.
Results:
Under condition of visual acuity 1.5 data of the depth of focus were 0.38 D, 0.64 D, and 1.92 D for pupil diameter 5.0 mm, 3.0 mm, and 1.0 mm, respectively. Visual acuity 1.0 is characterized by larger value and visual acuity 2.0 by lesser value of the depth of focus.
Conclusions:
Depth of focus and normal levels of visual acuity have a close relation: the higher the visual acuity, the smaller the depth of focus.
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