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Planar patch-clamp force microscopy on living cells.

Evren Pamir1, Michael George, Niels Fertig

  • 1Center for Nano Science, Ludwig-Maximilians University, Amalienstr 54, 80799 Munich, Germany.

Ultramicroscopy
|October 16, 2007
PubMed
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This study combines patch-clamp electrophysiology with atomic force microscopy (AFM) to measure both mechanical and electrical properties of single cells. This novel technique allows for detailed analysis of cell behavior and membrane dynamics.

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Traditional methods often struggle to simultaneously assess mechanical and electrophysiological properties of single cells, especially non-adherent ones.
  • Atomic Force Microscopy (AFM) excels at mechanical probing, while patch-clamp is standard for electrical measurements.

Purpose of the Study:

  • To develop and validate a novel integrated system combining patch-clamp and AFM for simultaneous measurement of cellular mechanical and electrophysiological properties.
  • To demonstrate the capability of this system for analyzing voltage-induced membrane movement in living cells.

Main Methods:

  • Development of a planar patch-clamp chip from borosilicate glass to support and immobilize cells.
  • Integration of the patch-clamp chip with an AFM system for simultaneous mechanical and electrical measurements.

Related Experiment Videos

  • Whole-cell voltage clamp configuration used for electrophysiological recordings and mechanical probing.

    • Main Results:

    • Successfully immobilized non-adherent cells (HEK293 and Jurkat) for mechanical property measurements.
    • Achieved simultaneous measurement of electrophysiological and mechanical properties of single cells.
    • Quantified voltage-induced membrane movement, with results consistent with prior studies.

    Conclusions:

    • The combined patch-clamp and AFM technique provides a powerful new tool for studying cell mechanics and electrophysiology.
    • This integrated approach facilitates detailed investigation of cellular responses to electrical stimuli.
    • The system offers improved access to electrophysiologically controlled cellular probes with reduced vibrational noise.