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ButterflyBase: a platform for lepidopteran genomics.

Alexie Papanicolaou1, Steffi Gebauer-Jung, Mark L Blaxter

  • 1Institute for Evolutionary Biology, University of Edinburgh, King's Buildings, EH9 3JT, UK.

Nucleic Acids Research
|October 16, 2007
PubMed
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ButterflyBase is a new online portal for lepidopteran genomics, offering a unified resource for over 273,000 expressed sequence tags (ESTs) from 30 species. This database aids researchers in evolutionary biology and genomics by providing access to clustered gene sets and annotations.

Area of Science:

  • Genomics
  • Bioinformatics
  • Evolutionary Biology

Background:

  • Lepidoptera represent a significant insect group with diverse research communities.
  • Existing genomic resources for Lepidoptera are fragmented, lacking a centralized access point.
  • The growing volume of genomic data necessitates a unified resource for analysis.

Purpose of the Study:

  • To introduce ButterflyBase, a novel, unified online portal for lepidopteran genomics.
  • To provide researchers with a comprehensive platform for accessing and analyzing lepidopteran genomic data.
  • To support various research needs, including molecular marker development and phylogenetic analysis.

Main Methods:

  • Clustering of over 273,000 expressed sequence tags (ESTs) from more than 30 Lepidoptera species.

Related Experiment Videos

  • Generation of unigene sets and protein translations.
  • Annotation of clusters and translations using BLAST similarity, Gene Ontology (GO), Enzyme Classification (EC), and Kyoto Encyclopaedia of Genes and Genomes (KEGG) terms.
  • Implementation of similarity search tools like BLAST and MS-BLAST.
  • Main Results:

    • Creation of stable unigene sets and robust protein translations for lepidopteran species.
    • Comprehensive annotation of genomic data, facilitating functional and evolutionary studies.
    • Development of a searchable database supporting diverse research applications.

    Conclusions:

    • ButterflyBase serves as a crucial, unified resource for the lepidopteran research community.
    • The platform enhances capabilities for molecular marker development, orthologue prediction, and evolutionary process studies.
    • Future expansion will incorporate additional genomic, ecological, and mapping data.