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Updated: Jul 10, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Microelectrospray interface with coaxial sheath flow for high-resolution capillary electrophoresis/mass spectrometry
Masahiko Hashimoto1, Yasushi Ishihama, Masaru Tomita
1Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0017, Japan.
Abstract:
We have fabricated a coaxial sheath liquid flow microelectrospray ionization (microESI) interface for capillary electrophoresis coupled with mass spectrometry (CE/MS). The ESI interface, which features a reduced probe diameter (130 microm i.d. x 174 microm o.d.) with a nebulizer-free format, can relatively easily electrospray a large amount of make-up sheath liquid (5-10 microL/min) over the long term (more than 80 runs) with a high degree of stability. The interface also provides higher separation qualities and improved detection sensitivities compared with a conventional ion spray (IS) interface.
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