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Updated: Jul 10, 2026

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
Published on: May 1, 2013
Ethidium monoazide and propidium monoazide for elimination of unspecific DNA background in quantitative universal
Ingeborg Hein1, Wilfried Schneeweiss, Christian Stanek
1Institute of Milk Hygiene, Milk Technology and Food Science, Department of Veterinary Public Health and Food Science, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria. ingeborg.hein@vu-wien.ac.at
Abstract:
Unspecific background DNA in quantitative universal real-time PCR utilizing a hydrolysis probe was completely suppressed by the addition of EMA or PMA to the PCR mix via cross-linking of the dyes to DNA during 650 W visible light exposure. The proposed procedure had no effect on the sensitivity of the real-time PCR reaction.
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