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[NgR expression in oligodendrocyte precursor cells and its changes after oxygen & glucose deprivation in neonatal
Jun Tang1, Yu-Jia Yao, Lin Zhong
1Department of Pediatrics, West China Second University Hospital, Chengdu 610041, China.
Objective:
This study examined the NgR expression in oligodendrocyte precursor cells (OLPs) and its changes after oxygen & glucose deprivation (OGD) in order to explore the role of NgR expression in the regeneration of OLPs after OGD in neonatal rats.
Methods:
The OLPs from 2-day-old neonatal rats were separated by improved separation and purification through agitation and then cultured in chemically defined medium. OLPs OGD model was prepared using the medium consisting of Na2S2O4 and Earle's fluid in vitro. Immunofluorescence assay was applied to identify the OLPs with its specific antibodies such as A2B5, O4 and O1. Western blot was used to detect the NgR expression in OLPs 10 and 30 minutes after OGD. The livability rate of cells was detected by MTT.
Results:
NgR expression was found in both the cell body and the prominence of purified OLPs. NgR expression in OLPs increased significantly 10 and 30 minutes after OGD compared with that in OLPs without OGD (controls, P < 0.05). MTT showed that the livability rate of OLPs at 30 minutes following OGD was significantly lower than that of controls (65.97+/-3.69% vs 97.17+/-6.88%, P < 0.05).
Conclusions:
NgR is expressed in both the cell body and the prominence of OLPs. NgR expression increases while cell livability decreases following OGD, suggesting that NgR may play a role in the inhibition of regeneration of OLPs.
