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Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
Published on: February 14, 2019
Evaluation of a glucose sensing antibody using weak affinity chromatography
Henrik A Engström1, Reine Johansson, Per Koch-Schmidt
1School of Pure and Applied Natural Sciences, University of Kalmar, SE-391 82 Kalmar, Sweden.
Biomedical Chromatography : BMC
|October 17, 2007
Summary
Researchers evaluated monoclonal antibody 3F1E8-A2 for continuous glucose monitoring. This antibody shows high specificity for glucose with a dissociation constant suitable for biosensor applications.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Immunology
Background:
- Continuous monitoring of endogenous molecules, like glucose, is crucial for managing diabetes mellitus.
- Developing effective recognition molecules with fast dissociation rates and millimolar dissociation constants is key for dynamic biosensor responses.
Purpose of the Study:
- To evaluate the suitability of monoclonal antibody (mAb) 3F1E8-A2 for continuous glucose sensing applications.
- To characterize the affinity and specificity of mAb 3F1E8-A2 towards glucose and other monosaccharides.
Main Methods:
- Generation of mAb 3F1E8-A2 by immunizing mice with alpha1,6-glucose polymer.
- Immobilization of the mAb onto macroporous silica.
- Evaluation of interactions using weak affinity chromatography (WAC) and competitive WAC.
Main Results:
- Monoclonal antibody 3F1E8-A2 demonstrated higher specificity for glucose over other monosaccharides.
- The dissociation constant (Kd) of mAb 3F1E8-A2 towards glucose was determined to be 18.8 ± 2.6 mM.
Conclusions:
- mAb 3F1E8-A2 exhibits favorable characteristics, including high glucose specificity and a millimolar dissociation constant.
- This antibody shows significant potential for use in future continuous glucose monitoring sensor development.
