Amperometric immunosensor for detecting Schistosoma mansoni antibody

Area of Science:

• Biomaterials Science
• Analytical Chemistry
• Immunotechnology

Background:

• Schistosoma mansoni infections require sensitive diagnostic tools.
• Existing immunosensors face challenges in sensitivity and stability.
• Development of robust platforms for antibody detection is crucial.

Purpose of the Study:

• To develop a novel immunosensor for detecting anti-apyrase antibodies of Schistosoma mansoni.
• To create a surface modification strategy for graphite-epoxy resin composites.
• To validate the immunosensor's performance using amperometric immunoassays.

Main Methods:

• Fabrication of a graphite powder-epoxy resin composite immunosensor.
• Surface modification via oxidation, silanization with 3-aminopropyltriethoxysilane, and glutaraldehyde activation.
• Characterization using contact angle microscopy and scanning electron microscopy.
• Validation through amperometric immunoassays for Schistosoma mansoni.

Main Results:

• Successful development of a modified conductive composite surface with amino and aldehyde groups.
• Demonstrated ability to immobilize proteins and detect anti-apyrase antibodies.
• Analyzed the influence of pH and incubation time on amperometric signals.
• Observed a direct correlation between amperometric signals and protein concentration.

Conclusions:

• The developed surface modification strategy is effective for creating a stable immunosensor platform.
• This method enables direct protein immobilization for immunosensor and protein array applications.
• The immunosensor shows promise for the sensitive detection of Schistosoma mansoni antibodies.