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Mononucleotide repeats are an abundant source of length variants in mouse genomic DNA
T J Aitman1, C M Hearne, M A McAleer
1Nuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford, UK.
Abstract:
Microsatellite sequences, such as dinucleotide repeats, show a high degree of polymorphism in eukaryotic DNA. These sequences are convenient as genetic markers and can be analyzed by the polymerase chain reaction (PCR). We have assessed the frequency of length variants in 18 mononucleotide repeats in mouse DNA and find that the variability is similar to that reported for dinucleotide repeats. Nine of the 18 repeat sequences (50%) have three or more alleles in the strains tested. Ten of these repeat sequences have been mapped using strain distribution patterns (SDPs) in recombinant inbred (RI) strains.