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Generation of conditional knockout alleles for PDGF-C

Xiaoli Wu1, Hao Ding

  • 1Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada.

Genesis (New York, N.Y. : 2000)
|October 18, 2007
PubMed

Insights

Platelet-derived growth factor-C (PDGF-C) is crucial for embryonic development, as its absence causes perinatal lethality. New conditional alleles of the Pdgf-c gene enable detailed study of PDGF-C

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Platelet-derived growth factor-C (PDGF-C) is a key signaling molecule in the PDGF family.
  • PDGF-C interacts with PDGF receptor (PDGFR)-alphaalpha and alphabeta dimers to regulate cellular functions.
  • PDGF-C deficiency leads to perinatal lethality, characterized by cleft palate formation.

Purpose of the Study:

  • To further characterize the cellular functions of PDGF-C during embryonic and postnatal development.
  • To generate conditional alleles of the Pdgf-c gene for precise spatial and temporal gene manipulation.
  • To establish an efficient method for creating conditional mouse alleles.

Main Methods:

  • Generation of two conditional alleles for the Pdgf-c gene, flanking exon 5 with loxP sites.
  • Utilizing global Cre-mediated excision to delete the floxed exon 5.
  • Phenotypic analysis of resulting PDGF-C deficient embryos.

Main Results:

  • Global Cre-mediated deletion resulted in a complete loss of PDGF-C expression.
  • Embryonic defects observed were identical to those of PDGF-C null embryos, including cleft palate.
  • The generated conditional alleles serve as valuable tools for studying PDGF-C roles.

Conclusions:

  • Conditional alleles of Pdgf-c are essential genetic tools for dissecting PDGF-C's roles in development and adult tissues.
  • These alleles facilitate the study of PDGF-C's spatial and temporal functions.
  • An efficient approach for creating mouse conditional alleles was successfully demonstrated.

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