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Related Experiment Video

Updated: Jul 10, 2026

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

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Published on: November 2, 2011

Quantifying interactions between cell receptors and adhesion ligand-modified polymers in solution.

Kuen Yong Lee1, Hyun Joon Kong, David J Mooney

  • 1Department of Bioengineering, Hanyang University, Seoul 133-791, South Korea. leeky@hanyang.ac.kr

Macromolecular Bioscience
|October 18, 2007
PubMed
Summary

Fluorescence resonance energy transfer (FRET) and rheology reveal specific cell-polymer interactions. This method aids in designing cell carriers for tissue engineering and drug delivery.

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Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell
14:09

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Published on: August 4, 2015

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Cell Biology
  • Tissue Engineering

Background:

  • Understanding cell-polymer interactions is crucial for developing advanced biomaterials.
  • Existing methods may not fully capture the dynamic nature of these interactions in solution.
  • Tailoring polymeric carriers requires precise control over cell-material interfaces.

Purpose of the Study:

  • To investigate specific interactions between cells and cell-interactive polymers in solution.
  • To evaluate the utility of fluorescence resonance energy transfer (FRET) and rheology for studying these interactions.
  • To explore the potential of this approach for designing biomaterials in tissue engineering.

Main Methods:

  • Utilized fluorescence resonance energy transfer (FRET) by labeling cells with rhodamine and polymers with fluorescein.
  • Employed rheological measurements to assess the structural changes in cell/polymer mixtures.
  • Compared FRET signals and rheological properties in the presence and absence of RGD-containing polymers.

Main Results:

  • A significant decrease in green fluorescence emission was observed, indicating FRET and specific cell-polymer binding.
  • Rheological measurements confirmed the formation of ordered structures in cell/polymer mixtures due to these interactions.
  • The FRET method provided both qualitative and quantitative insights into cell-polymer interactions.

Conclusions:

  • FRET is a valuable technique for studying cell-polymer interactions in solution.
  • Specific cell-polymer interactions lead to the formation of ordered structures, confirmed by rheology.
  • This approach can guide the design of polymeric carriers for cells and biological drugs in tissue engineering.