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A rapid CE-potential gradient detection method for determination of quinolones
Yongxia Fan1, Xiao Gan, Shuo Li
1College of Chemistry, Beijing Normal University, Beijing, PR China.
Electrophoresis
|October 18, 2007
Summary
A new capillary electrophoresis method with potential gradient detection rapidly separates four quinolones. This technique accurately determines these antibiotics in pharmaceutical formulations and spiked milk samples.
Area of Science:
- Analytical Chemistry
- Separation Science
- Pharmaceutical Analysis
Background:
- Quinolones are important antibiotics with potential applications in human and veterinary medicine.
- Accurate and rapid analytical methods are crucial for quality control and monitoring of pharmaceutical formulations and food products.
Purpose of the Study:
- To develop and validate a rapid capillary electrophoresis (CE) method coupled with potential gradient detection (PGD) for the separation and quantification of four quinolone antibiotics.
- To apply the developed method for the determination of ofloxacin (OFL) in pharmaceutical formulations.
- To couple the CE-PGD method with liquid-liquid extraction (LLE) for the analysis of quinolones in milk samples.
Main Methods:
- Capillary electrophoresis (CE) separation using a fused-silica capillary with a Tris-phosphoric acid buffer at pH 8.9.
- Potential gradient detection (PGD) for enhanced sensitivity and selectivity.
- Liquid-liquid extraction (LLE) using dichloromethane and hexane for sample preparation of milk matrices.
- Method validation by comparing results with High-Performance Liquid Chromatography (HPLC).
Main Results:
- Rapid separation of four quinolones (enoxacin, ofloxacin, fleroxacin, pazufloxacin) within 2.8 minutes.
- Good linearity (r(2) >= 0.985) was achieved for the separation.
- Successful determination of OFL in a pharmaceutical formulation.
- Effective enrichment and purification of quinolones from milk samples using LLE, with recoveries ranging from 77-106%.
- Low detection limits for quinolones using LLE-CE-PGD, ranging from 23 to 65 ng/mL.
Conclusions:
- The developed CE-PGD method offers a rapid, sensitive, and reliable approach for quinolone analysis.
- The method is suitable for determining quinolones in pharmaceutical products and complex matrices like milk after LLE.
- The results demonstrate good agreement with HPLC, validating the proposed CE-PGD technique.
