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Updated: Jul 10, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
[Optimization of manninotriose preparation from stachoyse]
Wei-hong Wu1, Xue-sen Wen, Yu Zhao
1School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. weihong204@163.com
Objective:
To optimize the processing of mannatriose preparation from stachyose by acidolysis.
Methods:
An orthogonal experiment was performed to select a better condition of acidolysis. Fructoes and stachyose was determined by HPLC.
Results:
Among the factors set in the present work, temperature and time have little influence on the acidolysis, while the effect of pH value was significant (P < 0.05). The best processing was selected as heating stachosy solution for 12h at pH 2.5 and 90 degrees C.
Conclusion:
It is practicable to prepare manninotriose from stachyoses by acidolysis.
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