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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
Published on: September 28, 2022
EBV detection in HIV-related oral plasmablastic lymphoma
K L Ferrazzo1, R A Mesquita, A T T Aburad
1Department of Oral Pathology, University of São Paulo, São Paulo, SP, Brazil. kivialinhares@uol.com.br
Oral Diseases
|October 20, 2007
Summary
Epstein-Barr virus (EBV) was detected in most cases of oral HIV-related plasmablastic lymphoma (PBL), suggesting a role in its development. Human herpesvirus-8 (HHV-8) was not found in these aggressive B cell neoplasms.
Area of Science:
- Oncology
- Virology
- Immunology
Background:
- Plasmablastic lymphoma (PBL) is an aggressive B cell neoplasm.
- It is the second most common malignancy associated with human immunodeficiency virus (HIV).
- Epstein-Barr virus (EBV) has been implicated in PBL development.
Purpose of the Study:
- To assess the presence of EBV in oral HIV-related PBL.
- To investigate the controversial association of Human herpesvirus-8 (HHV-8) with these tumors.
Main Methods:
- DNA extraction and polymerase chain reaction (PCR) for EBV detection.
- In situ hybridization (ISH) for EBV.
- Immunohistochemical analysis for diagnosis confirmation and HHV-8 detection.
Main Results:
- EBV was detected in 7 out of 9 evaluable cases of oral HIV-related PBL via PCR.
- All 5 cases tested using ISH showed positive signals for EBV.
- All 11 cases were negative for HHV-8.
Conclusions:
- The high prevalence of EBV supports its direct role in the pathogenesis of HIV-related PBL.
- These findings are crucial for managing HIV patients with this malignancy.
