Related Experiment Video

Updated: Jul 10, 2026

Phenol Red Thread-based Sampling Procedure for Untargeted Tear Fluid Lipidomics in Biomarker Discovery
08:16

Phenol Red Thread-based Sampling Procedure for Untargeted Tear Fluid Lipidomics in Biomarker Discovery

Published on: December 12, 2025

Evidence for internal and external binding sites on human tear lipocalin

Oktay K Gasymov1, Adil R Abduragimov, Ben J Glasgow

  • 1Department of Pathology, UCLA School of Medicine, Jules Stein Eye Institute, 100 Stein Plaza, Rm# B269, Los Angeles, CA 90095, USA.

Archives of Biochemistry and Biophysics
|October 20, 2007
PubMed

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Conserved Binding Sites01:49

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Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
Ligand Binding Sites02:40

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
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The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium: