Layer-by-Layer adsorption of biocompatible polyelectrolytes onto dexamethasone aggregates

Using layer-by-layer technology in drug delivery systems is advantageous because of its high precision, mild assembly conditions, and ease of use. In this study, we investigate the use of such a system using a micronized dexamethasone core as the building template. Dexamethasone was chosen because of its hydrophobic structure and role as a cellular differentiation factor. Structural characterization of the assembled structures shows particle size distribution between 3-10 mum with 20% more dissolution than free drug crystals. Additionally, as a measure of drug activity post-encapsulation, in vitro cell culture studies were performed. We suggest that the polyelectrolyte coatings enhance release and augment production of extracellular matrix proteins aggrecan and collagen II.