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Subsurface bioimaging using angular domain optical backscattering illumination.

F Vasefi1, P K Y Chan, B Kaminska

  • 1Comput. & Integrative Bio-Eng. Res., Simon Fraser Univ., Burnaby, BC Canada V5A 1S6.

Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
|October 20, 2007
PubMed
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Backscattering angular domain imaging uses micromachined collimators to detect photons in scattering media. This technique improves imaging depth and scattered-to-ballistic photon ratios, even in highly scattering environments.

Area of Science:

  • Biomedical Optics
  • Optical Imaging
  • Scattering Media

Background:

  • Coherence and time domain optical tomography detect shortest path photons in scattering media.
  • Backscattering angular domain imaging offers an alternative approach for imaging within scattering media.

Purpose of the Study:

  • To evaluate the effectiveness of backscattering angular domain imaging using micromachined collimators.
  • To assess the imaging capability in scattering media at various depths and scattered-to-ballistic photon ratios.

Main Methods:

  • Utilized micromachined semicircular silicon collimator channels as angular filters.
  • Employed front-side illumination to observe phantom test objects in scattering media up to 3 mm deep.
  • Investigated the impact of carbon coating on collimator reflectivity using a sputtering system.

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Main Results:

  • Successfully imaged phantom test objects in scattering media up to 3 mm deep.
  • Achieved effective scattered-to-ballistic photon ratios ranging from 1:1 to over 3E12:1.
  • Demonstrated the potential of carbon coating to reduce internal reflectivity of collimators.

Conclusions:

  • Backscattering angular domain imaging is effective for deep imaging in highly scattering media.
  • Micromachined collimators with reduced reflectivity enhance imaging performance.
  • This technique shows promise for applications requiring visualization within turbid environments.