Utlization of human expert techniques for detection of low-abundant peaks in high-resolution mass spectra
Grzegorz M Boratyn1, Michael L Merchant, Jon B Klein
1Kidney Disease Program and Clinical Proteomics Center, Louisville Univ., K, USA. greg.boratyn@luisville.edu
Abstract:
Interpretation and classification of mass spectra is usually performed using a list of peaks as their mathematical representation. This fact makes peak detection a bottleneck of mass spectra analysis, since quality and biological relevance of any results strongly depends on the accuracy of peak detection process. Many algorithms utilize intensity to differentiate between peaks and noise and thus bias the detection process to the high abundant peaks. However important information may also be contained in the lower-intensity peaks that are more difficult to discover. We present an algorithm specifically designed for detection of low-abundant peaks.
Related Concept Videos
High-Resolution Mass Spectrometry (HRMS)
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Tandem Mass Spectrometry
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry
Mass Spectrum: Interpretation

