Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Geometry and dynamics of annealed optimization in the coherent Ising machine with hidden and planted solutions.

Physical review. E·2026
Same author

Acute Epstein-Barr Virus-Related Myopericarditis in an Immunocompetent Young Adult.

Cureus·2025
Same author

Implementation and Evaluation of a Longitudinal Flipped-Classroom Point-of-Care Ultrasound Curriculum at an Internal Medicine Residency Program.

Journal of medical education and curricular development·2023
Same author

Ultra-broadband mid-infrared generation in dispersion-engineered thin-film lithium niobate.

Optics express·2022
Same author

Whistling Scrotum: An Unusual Presentation of Pneumomediastinum in the Setting of an Open Scrotal Wound.

The American journal of case reports·2022
Same author

Longitudinal Changes in Spirometry in Deployed Air Force Firefighters.

Journal of occupational and environmental medicine·2021

Related Experiment Video

Updated: Jul 10, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Quantum dot photon statistics measured by three-dimensional particle tracking.

Kevin McHale1, Andrew J Berglund, Hideo Mabuchi

  • 1Physical Measurement and Control 266-33, California Institute of Technology, Pasadena, California 91125, USA. mchale@caltech.edu

Nano Letters
|October 24, 2007
PubMed
Summary
This summary is machine-generated.

We developed a new instrument for single fluorescent particle analysis. This tool measures photon antibunching and tracks particle movement, revealing insights into quantum dot behavior in solution.

More Related Videos

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Related Experiment Videos

Last Updated: Jul 10, 2026

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

Area of Science:

  • Single-molecule spectroscopy
  • Quantum dot characterization
  • Nanoparticle dynamics

Background:

  • Single fluorescent particles exhibit complex behaviors like intermittency and fluorescence lifetime variations.
  • Understanding these behaviors is crucial for applications in quantum information and bioimaging.
  • Existing methods often lack the precision to simultaneously track particle motion and analyze fluorescence at the single-molecule level.

Purpose of the Study:

  • To develop and validate a novel instrument for simultaneous 3D Brownian motion tracking and correlation spectroscopy of single fluorescent particles.
  • To investigate photon antibunching and fluorescence lifetime heterogeneity in single quantum dots (QDs) in solution.
  • To explore the effect of 2-mercaptoethanol on QD fluorescence intermittency.

Main Methods:

  • Development of a real-time feedback instrument for 3D particle tracking.
  • Application of correlation spectroscopy to single CdSe/ZnS quantum dots in aqueous solution.
  • Measurement of photon antibunching and fluorescence lifetime distributions.
  • Analysis of fluorescence intermittency over time scales from milliseconds to seconds.

Main Results:

  • First measurements of photon antibunching (around 10 ns) for single fluorophores freely diffusing in solution.
  • Observation of significant fluorescence lifetime heterogeneity within the studied quantum dot sample.
  • Demonstration that 2-mercaptoethanol suppresses short time-scale intermittency (1 ms to 1 s) by reducing off-state duration.

Conclusions:

  • The developed instrument enables unprecedented simultaneous analysis of single-particle dynamics and fluorescence.
  • Single quantum dots exhibit intrinsic fluorescence lifetime heterogeneity and photon antibunching.
  • 2-mercaptoethanol effectively mitigates fluorescence intermittency in quantum dots, enhancing their stability for applications.